biotin mouse anti rat igg 1 Search Results


biotin rat igg2a  (Cedarlane)
85
Cedarlane biotin rat igg2a
Biotin Rat Igg2a, supplied by Cedarlane, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anticd25  (Cedarlane)
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Cedarlane anticd25
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biotinylated rat anti cd5  (Cedarlane)
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Cedarlane biotinylated rat anti cd5
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biotinylated anti mouse c1q  (Cedarlane)
92
Cedarlane biotinylated anti mouse c1q
A ) Total number of infiltrating CD11b + Ly6G high neutrophils recovered from the peritoneal cavity of J h t -/- Ggta1 -/- mice, either naive (dark gray circles; n=3) or 3 hours after injection of E. faecalis , unopsonized (light gray circles; n=2), or opsonized with IgG from Ggta1 + / + (white circles; n=3) or Ggta1 -/- (black circles; n=3) mice; 1 experiment. B) Total number of bacteria-containing neutrophils in the same samples as in (A). C ) IgG-dependent phagocytosis index calculated as a ratio of bacteria-containing neutrophils in IgG-opsonized groups over the unopsonized group, in the same samples as in (A). D-E) Detection of αGal in IgG from Ggta1 + / + and Ggta1 -/- mice, using D) BSI-B4 lectin and E ) Anti-αGal M86 mAb. SDS gel is shown as loading control. Representative of 6 independent IgG preparations. F ) Relative binding to FcγRIV and G ) Anti-IgG by Ggta1 + / + (n=6) and Ggta1 -/- (n=6) purified IgG. H ) Relative binding to mouse <t>C1q</t> by Ggta1 + / + (n=7) and Ggta1 -/- (n=3) purified IgG, where n corresponds to independent IgG preparations. Data is representative of 1-3 independent experiments. Symbols in (A, B, C) are individual mice. Red lines (A, B, C) are mean values. Error bars in (A, B, C) correspond to SD and in (F, G, H) to SEM. P values in (A) calculated using a one-tailed Kruskal-Wallis test with Dunn’s correction for multiple comparisons, in (B, C) using Mann-Whitney test and in (F-H) using 2-Way ANOVA with Sidak’s multiple comparison test. *P < 0.05; ns: not significant.
Biotinylated Anti Mouse C1q, supplied by Cedarlane, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated rat anti mac 3  (Cedarlane)
88
Cedarlane biotinylated rat anti mac 3
A ) Total number of infiltrating CD11b + Ly6G high neutrophils recovered from the peritoneal cavity of J h t -/- Ggta1 -/- mice, either naive (dark gray circles; n=3) or 3 hours after injection of E. faecalis , unopsonized (light gray circles; n=2), or opsonized with IgG from Ggta1 + / + (white circles; n=3) or Ggta1 -/- (black circles; n=3) mice; 1 experiment. B) Total number of bacteria-containing neutrophils in the same samples as in (A). C ) IgG-dependent phagocytosis index calculated as a ratio of bacteria-containing neutrophils in IgG-opsonized groups over the unopsonized group, in the same samples as in (A). D-E) Detection of αGal in IgG from Ggta1 + / + and Ggta1 -/- mice, using D) BSI-B4 lectin and E ) Anti-αGal M86 mAb. SDS gel is shown as loading control. Representative of 6 independent IgG preparations. F ) Relative binding to FcγRIV and G ) Anti-IgG by Ggta1 + / + (n=6) and Ggta1 -/- (n=6) purified IgG. H ) Relative binding to mouse <t>C1q</t> by Ggta1 + / + (n=7) and Ggta1 -/- (n=3) purified IgG, where n corresponds to independent IgG preparations. Data is representative of 1-3 independent experiments. Symbols in (A, B, C) are individual mice. Red lines (A, B, C) are mean values. Error bars in (A, B, C) correspond to SD and in (F, G, H) to SEM. P values in (A) calculated using a one-tailed Kruskal-Wallis test with Dunn’s correction for multiple comparisons, in (B, C) using Mann-Whitney test and in (F-H) using 2-Way ANOVA with Sidak’s multiple comparison test. *P < 0.05; ns: not significant.
Biotinylated Rat Anti Mac 3, supplied by Cedarlane, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cl005b  (Cedarlane)
93
Cedarlane cl005b
A ) Total number of infiltrating CD11b + Ly6G high neutrophils recovered from the peritoneal cavity of J h t -/- Ggta1 -/- mice, either naive (dark gray circles; n=3) or 3 hours after injection of E. faecalis , unopsonized (light gray circles; n=2), or opsonized with IgG from Ggta1 + / + (white circles; n=3) or Ggta1 -/- (black circles; n=3) mice; 1 experiment. B) Total number of bacteria-containing neutrophils in the same samples as in (A). C ) IgG-dependent phagocytosis index calculated as a ratio of bacteria-containing neutrophils in IgG-opsonized groups over the unopsonized group, in the same samples as in (A). D-E) Detection of αGal in IgG from Ggta1 + / + and Ggta1 -/- mice, using D) BSI-B4 lectin and E ) Anti-αGal M86 mAb. SDS gel is shown as loading control. Representative of 6 independent IgG preparations. F ) Relative binding to FcγRIV and G ) Anti-IgG by Ggta1 + / + (n=6) and Ggta1 -/- (n=6) purified IgG. H ) Relative binding to mouse <t>C1q</t> by Ggta1 + / + (n=7) and Ggta1 -/- (n=3) purified IgG, where n corresponds to independent IgG preparations. Data is representative of 1-3 independent experiments. Symbols in (A, B, C) are individual mice. Red lines (A, B, C) are mean values. Error bars in (A, B, C) correspond to SD and in (F, G, H) to SEM. P values in (A) calculated using a one-tailed Kruskal-Wallis test with Dunn’s correction for multiple comparisons, in (B, C) using Mann-Whitney test and in (F-H) using 2-Way ANOVA with Sidak’s multiple comparison test. *P < 0.05; ns: not significant.
Cl005b, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti rat icam  (Cedarlane)
90
Cedarlane anti rat icam
A ) Total number of infiltrating CD11b + Ly6G high neutrophils recovered from the peritoneal cavity of J h t -/- Ggta1 -/- mice, either naive (dark gray circles; n=3) or 3 hours after injection of E. faecalis , unopsonized (light gray circles; n=2), or opsonized with IgG from Ggta1 + / + (white circles; n=3) or Ggta1 -/- (black circles; n=3) mice; 1 experiment. B) Total number of bacteria-containing neutrophils in the same samples as in (A). C ) IgG-dependent phagocytosis index calculated as a ratio of bacteria-containing neutrophils in IgG-opsonized groups over the unopsonized group, in the same samples as in (A). D-E) Detection of αGal in IgG from Ggta1 + / + and Ggta1 -/- mice, using D) BSI-B4 lectin and E ) Anti-αGal M86 mAb. SDS gel is shown as loading control. Representative of 6 independent IgG preparations. F ) Relative binding to FcγRIV and G ) Anti-IgG by Ggta1 + / + (n=6) and Ggta1 -/- (n=6) purified IgG. H ) Relative binding to mouse <t>C1q</t> by Ggta1 + / + (n=7) and Ggta1 -/- (n=3) purified IgG, where n corresponds to independent IgG preparations. Data is representative of 1-3 independent experiments. Symbols in (A, B, C) are individual mice. Red lines (A, B, C) are mean values. Error bars in (A, B, C) correspond to SD and in (F, G, H) to SEM. P values in (A) calculated using a one-tailed Kruskal-Wallis test with Dunn’s correction for multiple comparisons, in (B, C) using Mann-Whitney test and in (F-H) using 2-Way ANOVA with Sidak’s multiple comparison test. *P < 0.05; ns: not significant.
Anti Rat Icam, supplied by Cedarlane, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti f4 80  (Cedarlane)
90
Cedarlane rat anti f4 80
A ) Total number of infiltrating CD11b + Ly6G high neutrophils recovered from the peritoneal cavity of J h t -/- Ggta1 -/- mice, either naive (dark gray circles; n=3) or 3 hours after injection of E. faecalis , unopsonized (light gray circles; n=2), or opsonized with IgG from Ggta1 + / + (white circles; n=3) or Ggta1 -/- (black circles; n=3) mice; 1 experiment. B) Total number of bacteria-containing neutrophils in the same samples as in (A). C ) IgG-dependent phagocytosis index calculated as a ratio of bacteria-containing neutrophils in IgG-opsonized groups over the unopsonized group, in the same samples as in (A). D-E) Detection of αGal in IgG from Ggta1 + / + and Ggta1 -/- mice, using D) BSI-B4 lectin and E ) Anti-αGal M86 mAb. SDS gel is shown as loading control. Representative of 6 independent IgG preparations. F ) Relative binding to FcγRIV and G ) Anti-IgG by Ggta1 + / + (n=6) and Ggta1 -/- (n=6) purified IgG. H ) Relative binding to mouse <t>C1q</t> by Ggta1 + / + (n=7) and Ggta1 -/- (n=3) purified IgG, where n corresponds to independent IgG preparations. Data is representative of 1-3 independent experiments. Symbols in (A, B, C) are individual mice. Red lines (A, B, C) are mean values. Error bars in (A, B, C) correspond to SD and in (F, G, H) to SEM. P values in (A) calculated using a one-tailed Kruskal-Wallis test with Dunn’s correction for multiple comparisons, in (B, C) using Mann-Whitney test and in (F-H) using 2-Way ANOVA with Sidak’s multiple comparison test. *P < 0.05; ns: not significant.
Rat Anti F4 80, supplied by Cedarlane, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotin anti mouse neutrophil monoclonal antibody  (Cedarlane)
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Cedarlane biotin anti mouse neutrophil monoclonal antibody
A ) Total number of infiltrating CD11b + Ly6G high neutrophils recovered from the peritoneal cavity of J h t -/- Ggta1 -/- mice, either naive (dark gray circles; n=3) or 3 hours after injection of E. faecalis , unopsonized (light gray circles; n=2), or opsonized with IgG from Ggta1 + / + (white circles; n=3) or Ggta1 -/- (black circles; n=3) mice; 1 experiment. B) Total number of bacteria-containing neutrophils in the same samples as in (A). C ) IgG-dependent phagocytosis index calculated as a ratio of bacteria-containing neutrophils in IgG-opsonized groups over the unopsonized group, in the same samples as in (A). D-E) Detection of αGal in IgG from Ggta1 + / + and Ggta1 -/- mice, using D) BSI-B4 lectin and E ) Anti-αGal M86 mAb. SDS gel is shown as loading control. Representative of 6 independent IgG preparations. F ) Relative binding to FcγRIV and G ) Anti-IgG by Ggta1 + / + (n=6) and Ggta1 -/- (n=6) purified IgG. H ) Relative binding to mouse <t>C1q</t> by Ggta1 + / + (n=7) and Ggta1 -/- (n=3) purified IgG, where n corresponds to independent IgG preparations. Data is representative of 1-3 independent experiments. Symbols in (A, B, C) are individual mice. Red lines (A, B, C) are mean values. Error bars in (A, B, C) correspond to SD and in (F, G, H) to SEM. P values in (A) calculated using a one-tailed Kruskal-Wallis test with Dunn’s correction for multiple comparisons, in (B, C) using Mann-Whitney test and in (F-H) using 2-Way ANOVA with Sidak’s multiple comparison test. *P < 0.05; ns: not significant.
Biotin Anti Mouse Neutrophil Monoclonal Antibody, supplied by Cedarlane, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti rat rt1 a  (Cedarlane)
85
Cedarlane anti rat rt1 a
A ) Total number of infiltrating CD11b + Ly6G high neutrophils recovered from the peritoneal cavity of J h t -/- Ggta1 -/- mice, either naive (dark gray circles; n=3) or 3 hours after injection of E. faecalis , unopsonized (light gray circles; n=2), or opsonized with IgG from Ggta1 + / + (white circles; n=3) or Ggta1 -/- (black circles; n=3) mice; 1 experiment. B) Total number of bacteria-containing neutrophils in the same samples as in (A). C ) IgG-dependent phagocytosis index calculated as a ratio of bacteria-containing neutrophils in IgG-opsonized groups over the unopsonized group, in the same samples as in (A). D-E) Detection of αGal in IgG from Ggta1 + / + and Ggta1 -/- mice, using D) BSI-B4 lectin and E ) Anti-αGal M86 mAb. SDS gel is shown as loading control. Representative of 6 independent IgG preparations. F ) Relative binding to FcγRIV and G ) Anti-IgG by Ggta1 + / + (n=6) and Ggta1 -/- (n=6) purified IgG. H ) Relative binding to mouse <t>C1q</t> by Ggta1 + / + (n=7) and Ggta1 -/- (n=3) purified IgG, where n corresponds to independent IgG preparations. Data is representative of 1-3 independent experiments. Symbols in (A, B, C) are individual mice. Red lines (A, B, C) are mean values. Error bars in (A, B, C) correspond to SD and in (F, G, H) to SEM. P values in (A) calculated using a one-tailed Kruskal-Wallis test with Dunn’s correction for multiple comparisons, in (B, C) using Mann-Whitney test and in (F-H) using 2-Way ANOVA with Sidak’s multiple comparison test. *P < 0.05; ns: not significant.
Anti Rat Rt1 A, supplied by Cedarlane, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti cd4  (Cedarlane)
86
Cedarlane rat anti cd4
A ) Total number of infiltrating CD11b + Ly6G high neutrophils recovered from the peritoneal cavity of J h t -/- Ggta1 -/- mice, either naive (dark gray circles; n=3) or 3 hours after injection of E. faecalis , unopsonized (light gray circles; n=2), or opsonized with IgG from Ggta1 + / + (white circles; n=3) or Ggta1 -/- (black circles; n=3) mice; 1 experiment. B) Total number of bacteria-containing neutrophils in the same samples as in (A). C ) IgG-dependent phagocytosis index calculated as a ratio of bacteria-containing neutrophils in IgG-opsonized groups over the unopsonized group, in the same samples as in (A). D-E) Detection of αGal in IgG from Ggta1 + / + and Ggta1 -/- mice, using D) BSI-B4 lectin and E ) Anti-αGal M86 mAb. SDS gel is shown as loading control. Representative of 6 independent IgG preparations. F ) Relative binding to FcγRIV and G ) Anti-IgG by Ggta1 + / + (n=6) and Ggta1 -/- (n=6) purified IgG. H ) Relative binding to mouse <t>C1q</t> by Ggta1 + / + (n=7) and Ggta1 -/- (n=3) purified IgG, where n corresponds to independent IgG preparations. Data is representative of 1-3 independent experiments. Symbols in (A, B, C) are individual mice. Red lines (A, B, C) are mean values. Error bars in (A, B, C) correspond to SD and in (F, G, H) to SEM. P values in (A) calculated using a one-tailed Kruskal-Wallis test with Dunn’s correction for multiple comparisons, in (B, C) using Mann-Whitney test and in (F-H) using 2-Way ANOVA with Sidak’s multiple comparison test. *P < 0.05; ns: not significant.
Rat Anti Cd4, supplied by Cedarlane, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated mouse igg1  (Cedarlane)
85
Cedarlane biotinylated mouse igg1
A ) Total number of infiltrating CD11b + Ly6G high neutrophils recovered from the peritoneal cavity of J h t -/- Ggta1 -/- mice, either naive (dark gray circles; n=3) or 3 hours after injection of E. faecalis , unopsonized (light gray circles; n=2), or opsonized with IgG from Ggta1 + / + (white circles; n=3) or Ggta1 -/- (black circles; n=3) mice; 1 experiment. B) Total number of bacteria-containing neutrophils in the same samples as in (A). C ) IgG-dependent phagocytosis index calculated as a ratio of bacteria-containing neutrophils in IgG-opsonized groups over the unopsonized group, in the same samples as in (A). D-E) Detection of αGal in IgG from Ggta1 + / + and Ggta1 -/- mice, using D) BSI-B4 lectin and E ) Anti-αGal M86 mAb. SDS gel is shown as loading control. Representative of 6 independent IgG preparations. F ) Relative binding to FcγRIV and G ) Anti-IgG by Ggta1 + / + (n=6) and Ggta1 -/- (n=6) purified IgG. H ) Relative binding to mouse <t>C1q</t> by Ggta1 + / + (n=7) and Ggta1 -/- (n=3) purified IgG, where n corresponds to independent IgG preparations. Data is representative of 1-3 independent experiments. Symbols in (A, B, C) are individual mice. Red lines (A, B, C) are mean values. Error bars in (A, B, C) correspond to SD and in (F, G, H) to SEM. P values in (A) calculated using a one-tailed Kruskal-Wallis test with Dunn’s correction for multiple comparisons, in (B, C) using Mann-Whitney test and in (F-H) using 2-Way ANOVA with Sidak’s multiple comparison test. *P < 0.05; ns: not significant.
Biotinylated Mouse Igg1, supplied by Cedarlane, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A ) Total number of infiltrating CD11b + Ly6G high neutrophils recovered from the peritoneal cavity of J h t -/- Ggta1 -/- mice, either naive (dark gray circles; n=3) or 3 hours after injection of E. faecalis , unopsonized (light gray circles; n=2), or opsonized with IgG from Ggta1 + / + (white circles; n=3) or Ggta1 -/- (black circles; n=3) mice; 1 experiment. B) Total number of bacteria-containing neutrophils in the same samples as in (A). C ) IgG-dependent phagocytosis index calculated as a ratio of bacteria-containing neutrophils in IgG-opsonized groups over the unopsonized group, in the same samples as in (A). D-E) Detection of αGal in IgG from Ggta1 + / + and Ggta1 -/- mice, using D) BSI-B4 lectin and E ) Anti-αGal M86 mAb. SDS gel is shown as loading control. Representative of 6 independent IgG preparations. F ) Relative binding to FcγRIV and G ) Anti-IgG by Ggta1 + / + (n=6) and Ggta1 -/- (n=6) purified IgG. H ) Relative binding to mouse C1q by Ggta1 + / + (n=7) and Ggta1 -/- (n=3) purified IgG, where n corresponds to independent IgG preparations. Data is representative of 1-3 independent experiments. Symbols in (A, B, C) are individual mice. Red lines (A, B, C) are mean values. Error bars in (A, B, C) correspond to SD and in (F, G, H) to SEM. P values in (A) calculated using a one-tailed Kruskal-Wallis test with Dunn’s correction for multiple comparisons, in (B, C) using Mann-Whitney test and in (F-H) using 2-Way ANOVA with Sidak’s multiple comparison test. *P < 0.05; ns: not significant.

Journal: bioRxiv

Article Title: A trade-off between resistance to infection and reproduction in primate evolution

doi: 10.1101/2020.07.10.186742

Figure Lengend Snippet: A ) Total number of infiltrating CD11b + Ly6G high neutrophils recovered from the peritoneal cavity of J h t -/- Ggta1 -/- mice, either naive (dark gray circles; n=3) or 3 hours after injection of E. faecalis , unopsonized (light gray circles; n=2), or opsonized with IgG from Ggta1 + / + (white circles; n=3) or Ggta1 -/- (black circles; n=3) mice; 1 experiment. B) Total number of bacteria-containing neutrophils in the same samples as in (A). C ) IgG-dependent phagocytosis index calculated as a ratio of bacteria-containing neutrophils in IgG-opsonized groups over the unopsonized group, in the same samples as in (A). D-E) Detection of αGal in IgG from Ggta1 + / + and Ggta1 -/- mice, using D) BSI-B4 lectin and E ) Anti-αGal M86 mAb. SDS gel is shown as loading control. Representative of 6 independent IgG preparations. F ) Relative binding to FcγRIV and G ) Anti-IgG by Ggta1 + / + (n=6) and Ggta1 -/- (n=6) purified IgG. H ) Relative binding to mouse C1q by Ggta1 + / + (n=7) and Ggta1 -/- (n=3) purified IgG, where n corresponds to independent IgG preparations. Data is representative of 1-3 independent experiments. Symbols in (A, B, C) are individual mice. Red lines (A, B, C) are mean values. Error bars in (A, B, C) correspond to SD and in (F, G, H) to SEM. P values in (A) calculated using a one-tailed Kruskal-Wallis test with Dunn’s correction for multiple comparisons, in (B, C) using Mann-Whitney test and in (F-H) using 2-Way ANOVA with Sidak’s multiple comparison test. *P < 0.05; ns: not significant.

Article Snippet: Plates were incubated with serially diluted mouse C1q (CompTech #M099), washed (5x, PBS/0.05% Tween-20), incubated with biotinylated anti-mouse C1q (Cedarlane #CL7501B, 1:50,000 vol/vol, PBS/1%BSA/0.01% Tween-20, 1 h, RT) and washed (5x, PBS/0.05% Tween-20).

Techniques: Injection, Bacteria, SDS-Gel, Control, Binding Assay, Purification, One-tailed Test, MANN-WHITNEY, Comparison